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KMID : 1134819960250060969
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Journal of the Korean Society of Food Science and Nutrition
1996 Volume.25 No. 6 p.969 ~ p.975
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Effect of Dietary Vitamin E on the Microsomal Mixed Function Oxidase System of Liver and Lung in Streptozotocin - induced Diabetic Rats
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Park Young-Ran
Rhee Soon-Jae
Lim Young-Suk
Joo Gil-Jae
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Abstract
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The purpose of this study was to investigate the effect of dietary vitamin E on microsomal mixed function oxidase system of liver and lung in streptozotocin(STZ) induced diabetic rats. Sprague-Dawley male rats weighing 140±10㎎ were randomly assigned to one control and three STZ-diabetic groups. Diabetic groups were divided into DM-0E(vitamin E free diet), DM-40E(40㎎ vitamin E ㎏/diet) and DM-400E(400㎎ vitamin E ㎏/diet) according to the level of vitamin E supplementation. Diabetes was experimentally induced by intravenous administration of 55㎎/㎏ b.w of STZ in citrate buffer(pH 4.3) after 4 week feeding of three experimental diets. Animals were sacrificed at the 6th day of diabetic state. The contents of cytochrome P_(450) in DM-0E, DM-40E and DM-400E groups of liver were increased by 162%, 150% and 56%, respectively, compared with that of control. Also the contents of cytochrome P_(450) in lung were similar to liver. The activities of cytochrome bs in DM-0E and DM-40E groups of the liver were increased by 70% and 53%, respectively, compared with that of control, but not in DM-400E group. The activities of bs in DM-0E, DM -40E and DM -400E groups of lung were signficantly increased. Activity of cytochrome P_(450) reductase in DM-0E, DM-40E of liver and lung were higher than that of control group, but the activity of DM-400E group was not different from that of control. The lipid peroxide values of DM-0E, DM-40E and DM-400E groups were 143%, 95% and 31% higher than those of control. It was concluded that dietary vitamin E had protective effects on lipid peroxidation accompanied with increased mixed function of oxidase activity in diabetic rats.
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KEYWORD
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diabetes, mixed function oxidase system, vitamin E
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